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Hplc working laboratory manual


Hplc working laboratory manual

Sample Injector : The injector can be a single injection or an automated injection system. An injector for an HPLC system should provide injection of the liquid sample within the range of mL of volume with high reproducibility and under high pressure (up to 4000.
Environmental Applications 1. Detection of phenolic compounds in drinking water. 2. Bio-monitoring of pollutants. Applications in Forensics 1. Quantification of drugs in biological samples. 2. Identification of steroids in blood, urine etc.
Data Collection Devices : Signals from the detector may be collected on chart recorders or electronic integrators that vary in complexity and in their ability to process, store and reprocess chromatographic data.
Pump : A pump aspirates the mobile phase from the solvent resorvoir and forces it through the systems column and detecter. Depending on a number of factors including column dimensions, particle size of the stationary phase, the flow rate and composition of the mobile phase.
High perfomance Liquid Chromatography High performance liquid chromatography (HPLC ) is basically a highly improved form of column liquid chromatography. Instead of a solvent being allowed to drip through a column under gravity, it is forced through under high pressures of up to 400 atmospheres.
That makes it much faster. All chromatographic separations, including HPLC operate under the same basic principle; separation of a sample into its constituent parts because of the difference in the relative affinities of different molecules for the mobile phase and the stationary phase used in.
3. Forensic analysis of textile dyes. 4. Determination of cocaine and other drugs of abuse in blood, urine etc. Food and Flavour 1. Measurement of Quality of soft drinks and water. 2.
Types of HPLC There are following variants of HPLC, depending upon the phase system (stationary) in the process : Normal Phase HPLC : This method separates analytes on the basis of polarity.
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Columns with internal diameters of less than 2 mm are often referred to as microbore columns. Ideally the temperature of the mobile phase and the column should be kept constant during an analysis.
It also aids in chemical separation and purification. The other applications of HPLC include : Pharmaceutical Applications 1. To control drug stability. 2. Tablet dissolution study of pharmaceutical dosages form. 3. Pharmaceutical quality control.


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